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KMID : 0363320140350030288
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Journal of Korean Oriental Internal Medicine
2014 Volume.35 No. 3 p.288 ~ p.297
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Antimicrobial Activity of Hwangryunhaedok-tang Extract against Keratitis
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Kim In-Soo
Seo Jin-Jong
Kim Kwang-Gon
Ha Dong-Ryong
Shin Min-Gu
Kim Eui-Su
Jeon Sang-Yun
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Abstract
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Objectives: The goal of this project was to measure the antibacterial effectiveness of Hwangryunhaedok-tang (barberry
root decoction for detoxification) and its constituents. All of the active ingredients including the final product were prepared
using high-pressure sterilization for use as medicinal eye drops. The varying forms of Hwangryunhaedok-tang (barberry root decoction for detoxification) were used to treat Staphylococcus epidermidis and Enterococcus faecalis which are found in keratitis.
Methods: The antibacterial effect was measured by observing the presence or absence of antimicrobial activity when treated with varying concentrations of Hwangryunhaedok-tang extract. The tests were performed using a dosage of 70 μl dosages of 100%, 50%, 10% and 1% the extracted solution by the minimum growth inhibitory concentration measurement. Antimicrobial activity was measured by examining the correlation between dosage strength and bacterial activity from 70 μl to 10 μl at the same concentration.
Results: 1. Hwangryunhaedok-tang (barberry root decoction for detoxification), Phellodendri Cortex, and gardenia didn't show any antimicrobial effects against S. aureus, S. epidermidis, or E. faecalis. 2. Barberry root showed antimicrobial effects against S. aureus and S. epidermidis depending on the levels of concentration but didn't show any antimicrobial effects against E. faecalis. 3. Skullcap showed antimicrobial effects against S. aureus and S. epidermidis when a dosage of 100% extract 70 μl was used. However, did not show any antimicrobial effects at all against E. faecalis.
Conclusions: Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents such as barberry
root, phellodendri cortex, skullcap, and gardenia, can be used as an alternative to antibiotic medicinal eye drops to treat keratitis. However, further research on effective uses of and efficient extraction methods are needed.
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KEYWORD
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Hwangryunhaedok-tang (huanglianjiedu-tang), Keratitis, S. aureus, S. epidermidis, Enterococcus faecalis
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